quick cip enzyme (New England Biolabs)

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New England Biolabs quick cip enzyme
Quick Cip Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quick+cip+enzyme/Quick+CIP/pm40914913-64-9-12
Average 99 stars, based on 1253 article reviews

quick cip enzyme - by Bioz Stars, 2026-09

99/100 stars

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Article Title: A Bioorthogonal Post-modification of N 6 -Isopentenyl Adenosine i 6 A RNA in Live Cells
Article Snippet: .. For the nucleoside analysis, 10 μL of the reaction mixture was mixed with nuclease P1 (1 μL, 0.2 U.μL−1, Sigma N8630-1VL, Merck KGaA, Darmstadt, Germany), NaCl (500mM, 2 μL), ZnCl2 (25 mM, 5 μL), and Nuclease-free water (25 μL), incubated at 42 °C for 2 h. Then Quick CIP enzyme (2 μL, 1U.μL−1, NEB M0290L, New England Biolabs, Ipswich, MA, USA) and CIP buffer (Alkaline Phosphatase Calf Intestinal), New England Biolabs, Ipswich, MA, USA (5 μL) were added to the reaction mixture and incubated at 37 °C for 1 h. The reaction mixture (48 μL) was analyzed on an HPLC system with a flow rate of 1 mL ·min−1 at room temperature. ..

Article Title: Cas9-targeted Nanopore sequencing rapidly elucidates the transposition preferences and DNA methylation profiles of mobile elements in plants.
Article Snippet: Transposable element insertions (TEIs) are an important source of genomic innovation by contributing to plant adaptation, speciation, and the production of new varieties.. The often large, complex plant genomes make identifying TEIs from short reads difficult and expensive.. Moreover, rare somatic insertions that reflect mobilome dynamics are difficult to track using short reads.

Article Title: Multiplexed optical barcoding and sequencing for spatial omics
Article Snippet: .. We then incubated the cells with phosphatase buffer composed of 1x rCutsmart buffer (NEB, B6004S), 0.1 % TX-100, 250 U/ml quick CIP enzyme (NEB, M0525S) for 20 min at 37 °C to remove any free 5’ phosphate groups that may have formed due to stray light and washed the samples with 1x PBS once and nuclease-free water once. ..

Article Title: capTEs enables locus-specific dissection of transcriptional outputs from reference and nonreference transposable elements.
Article Snippet: .. Three microliters of Quick CIP enzyme (NEB, M0525) and 3 μL of 10× Cutsmart buffer (NEB, B7204) were added to the eluate, and the mixture was incubated at 37 °C for 30 min for dephosphorylation. .. After inactivation of the phosphatase at 80 °C for 5 min, the DNA molecules were cut with 10 pmol of Cas9-gRNA complex (2.5 pmol each) at 37 °C for 15 min. For the removal of Cas9 protein from cleavage sites, the sample was digested with 1.5 μL of protease (Qiagen, 19155) for 10 min at 56 °C, followed by 70 °C for 15 min to inactivate protease. dA-tailing was carried out at 72 °C for 5 min with 1 μL of 10 mM dATP (NEB, N0440) and 5 U of Taq DNA polymerase (Vazyme, P101-d1-AC).

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quick cip enzyme (New England Biolabs)

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